Increased EV yield and bioactivity of matrix-bound nanovesicles compared to conditioned culture medium-derived extracellular vesicles
Aim: Extracellular vesicles (EVs) are nanoparticles secreted by cells for cell-cell communication functions, such as wound repair, but low yields often limit their use. Several isolation methods have been explored to increase EV yield from conditioned culture medium (liquid-EVs); however, few studies compare isolation techniques and yield between liquid-EVs and matrix-bound nanovesicles (MBVs), a…
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