Releasing Protein Cargo from Synthetic Cells by Coupling Cell-Free Expression to Osmotic Actuation
Macromolecular release from synthetic cells often relies on membrane pores or channels. We explored whether internally generated osmotic stress could increase membrane permeability and promote release. Using double-emulsion microfluidics, we encapsulated an Escherichia coli cell-free expression system in Pluronic L121 polymersomes to co-express the fluorescent protein mScarlet3 and the dextranase…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
