3D MERFISH reveals cell-type diversity generated by mRNA partitioning in spiralian embryogenesis
Cell type diversification during development is often attributed to differential gene transcription. Here, we show that, during Crepidula atrasolea embryogenesis, transcriptomically distinct cell types arise through differential distribution of pre-existing mRNAs. Combining immunofluorescence, live imaging, and whole-mount 3D spatial transcriptomics (MERFISH) of over 40 embryos, we show that many…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
