Expanding the Possibilities of Next-Generation Sequencing

Sequencing by expansion (SBX), the technology powering Roche's AXELIOS 1 platform, offers a novel approach to sequencing challenges posed by complex genomic features. This method physically expands the space between encoded bases before detection, creating a clearer signal for more accurate sequencing. SBX employs a polymerase that converts target DNA or RNA molecules into longer surrogate polymers, known as Xpandomers, which can be measured using a reusable complementary metal-oxide semiconductor (CMOS) sensor.

This design results in greater physical separation between encoded bases and enables high signal-to-noise reporters for precise single-molecule sequencing with near real-time base calling and analysis.

The AXELIOS 1 platform's flexibility extends beyond its chemistry, accommodating various research needs. Researchers can adjust batch sizes and read lengths according to their experimental requirements, with read lengths ranging from ~200bp to ~1500bp. The platform supports end-to-end, same-day whole genome sequencing (WGS) while providing near real-time data access during runs.

SBX has been successfully tested in various applications, including whole genome sequencing (WGS), RNA sequencing (RNA-seq), single-cell RNA sequencing (scRNA-seq), spatial analysis, and methylation studies. The AXELIOS 1 Platform sequences Xpandomer molecules generated from libraries prepared using the AXELIOS 1 DNA Library Prep Kit, with data analyzed using the no-cost, open-source XOOS bioinformatics analysis suite.

By combining a new sequencing chemistry with flexible read lengths, batch sizes, and research applications, AXELIOS 1 aims to expand the range of genomic questions researchers can address. As sequencing technology continues to evolve, these capabilities could enable scientists to investigate previously challenging genomic regions, ultimately building a more comprehensive foundation for biological discovery.