"Double-dipping" in testing for phenotypic differences with scRNA-Seq
For the purpose of differential expression (DE) analysis in single-cell RNA-sequencing (scRNA-Seq), phenotype differences between samples are often tested within specific cell types. Cell types are regularly imputed by clustering the same gene expression data which is later used for phenotype testing. This creates the potential for a "double-dipping" or post-selection inference problem resulting…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
