Autophagic flux is increased in peripheral blood mononuclear cells in atherosclerotic vascular disease and associates inversely with adverse cardiovascular events
Background: Autophagy is a homeostatic pathway supporting stress adaptation and is dysregulated in atherosclerosis. Its potential as a biomarker or therapeutic target in atherosclerotic vascular disease (ASVD) remains incompletely defined. We measured autophagic flux in peripheral blood mononuclear cells (PBMCs) from patients with peripheral arterial disease (PAD) or carotid stenosis (CS),…
Autophagic flux, a homeostatic pathway that supports stress adaptation, is found to be elevated in peripheral blood mononuclear cells (PBMCs) of individuals with atherosclerotic vascular disease (ASVD) compared to healthy controls. This finding suggests a potential link between increased autophagic flux and adverse cardiovascular events in ASVD patients.
The study, which involved 94 patients with PAD or carotid stenosis (CS) and 19 healthy controls, measured autophagic flux using a validated ex vivo chloroquine inhibition ELISA that quantifies LC3BII accumulation. The results showed that ASVD patients had a mean autophagic flux of 281.4 ng LC3BII/mg protein/h, significantly higher than the 182.3 ng in controls.
The study also explored the relationship between autophagic flux and major adverse cardiovascular events (MACE) and major adverse limb events (MALE).
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