Low-latency multicamera 3D tracking of insects with Braid
Advances in camera technology and computer vision techniques have allowed researchers to track animals in 3D in ways which previously were difficult or impossible. Many such 3D tracking tools make use of multiple cameras, but unfamiliarity with the principles and technology involved can make it difficult to employ such techniques. In this protocol, we describe Braid, open-source software for…
Recent advancements in camera technology and computer vision have enabled researchers to track animals in 3D, previously an arduous task. Multiple camera systems for 3D tracking exist, but unfamiliarity with these techniques can hinder their use. This protocol introduces Braid, an open-source software for real-time, multi-camera 3D tracking of insects.
Braid utilizes background-subtraction, allowing for object detection without the need for physical markers attached to the insect. The software generates low-latency 3D position estimates through Kalman filtering and nearest neighbor data association. The protocol provides comprehensive details on tracking freely flying bees within a flight arena using Braid.
This includes installation, camera configuration, setup, calibration, and operational instructions. The results demonstrate that Braid can achieve position estimates accurate to a remarkable level.
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