Multimodal optical imaging reveals spatial metabolic heterogeneity in the aging retina

Understanding how aging reshapes retinal metabolism requires methods that can resolve molecular and structural changes across the highly organized cellular layers of retina. Here, we applied a nonlinear multimodal imaging platform that integrates fluorescence lifetime imaging microscopy (FLIM), second-harmonic generation (SHG), hyperspectral stimulated Raman scattering (HS-SRS), and deuterium…

biorxiv.org ·23h ago

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