LIT (Layer-Wise Image Trajectories): In Situ Monitoring for Early Quality Prediction and Anomaly Detection in Acellular and Cell-Laden Two-Photon Polymerization

Two-photon polymerization (2PP) enables fabrication of hydrogel constructs with submicron, cell-scale resolution, but hydrogel-based bioinks are markedly more sensitive to process variability than conventional photoresists, and this sensitivity is further amplified when living cells are embedded in the resin. Post-processing evaluation, performed only after development, occurs too late to enable…

biorxiv.org ·14h ago

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