PKA can adopt structural or enzymatic roles depending on its anchoring site
Protein kinase A (PKA) is a central mediator of cAMP signaling whose spatial specificity is conferred by A-kinase anchoring proteins (AKAPs). However, functions of individual anchored PKA pools are difficult to resolve as current approaches cannot isolate anchoring at defined sites. Here, we develop a SpyCatcher-SpyTag-based replacement strategy enabling direct comparison of AKAP function with…
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