Host CD59 potentiates the type III secretion system in Yersinia pseudotuberculosis

The research explores the role of host protein CD59 in facilitating the type III secretion system (T3SS) of Yersinia pseudotuberculosis (Yptb). Previous studies have shown that CD59 is crucial for the translocation of Yptb T3SS effectors into the host cytosol. In this study, the researchers investigated how CD59 affects T3SS function and membrane dynamics.

They found that both low and high levels of CD59 reduced T3SS pore formation and Yop translocation into host cells. The depletion of CD59 also interfered with the rearrangement of lipid rafts and altered phosphatidylcholine levels, suggesting that CD59 modulates lipid microdomain dynamics. This work identifies CD59 as a key host factor supporting plasma membrane dynamics necessary for efficient T3SS translocon function.