SACHET: seeded contour tracking measures Rab conversion kinetics on individual endosomes in live cells
Membrane-labeled endosomes appear as hollow rings in a projection; they move, deform and exchange their coat, which population trackers (tools that detect every object of one kind and link the detections across frames) are not designed to follow. Here we introduce SACHET (Seeded Adaptive Contour & Hollow Endosome Tracking), a human-supervised Fiji tool that tracks one endosome at a time. On…
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