Systematic evaluation of transcript normalization methods and quantification pipelines across long-read sequencing platforms
Long-read RNA sequencing (lrRNA-seq) has transformed transcriptomics by enabling full-length isoform resolution, yet transcript quantification remains confounded by platform-dependent length biases and tool choices. While a quantification normalization step is common in short-read RNA-seq, this has not yet been extended to lrRNA-seq and the field lacks a systematic analysis of normalization…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
