Macrophages from Holstein and Normande cows respond differently to Escherichia coli and Streptococcus uberis
Macrophages, alongside mammary epithelial cells, are major contributors to the innate immune response triggered by bacterial invasion of the mammary gland. To investigate breed-specific immune mechanisms, we stimulated monocyte-derived macrophages from Holstein and Normande cattle with Escherichia coli and Streptococcus uberis, two major mastitis pathogens. We monitored bacterial survival using…
Macrophages derived from Holstein and Normande cows react differently when confronted by Escherichia coli and Streptococcus uberis, two prevalent mastitis-causing bacteria. Researchers stimulated monocyte-derived macrophages from both cow breeds using the two pathogens, then examined bacterial survival via [method] and gene expression through RNA-seq.
E. coli demonstrated superior survival six hours post-infection compared to S. uberis (p 1), with S. uberis only surviving 813 hours. Unique gene expression patterns were also noted; genes like CCL2 and CSF1 were specifically activated by S. uberis. Moreover, macrophages from Holstein and Normande cows exhibited divergent expression profiles.
After being exposed to E. coli or S. uberis, 185, 45, and 174 genes were differentially expressed at steady state or post-stimulation, respectively. These genes were linked to immune pathways. Consequently, the study reveals that Holstein and Normande macrophages mount a stronger defense against E. coli, while S. uberis triggers a distinct set of genes.
The breed-specific variations in immune responses could reveal underlying mechanisms that impact susceptibility to disease, suggesting the need for additional functional studies.
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