Nailpolish: Reference-free UMI deduplication and consensus read generation
Long-read sequencing captures full-length transcripts but suffers elevated error rates, and PCR duplicates can distort quantification unless accounted for with UMIs. We present Nailpolish, a fast reference-free tool that error-corrects and deduplicates UMI-tagged long reads by generating consensus sequences via partial order alignment, while separating false duplicates arising from UMI…
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