Metabolic tagging of adipose-derived stem cells for targeted modulation of regenerative potency within mineralized collagen scaffolds
Recent efforts to reconstruct critical-sized craniomaxillofacial defects using biomaterials have increasingly sought strategies to accelerate proangiogenic processes immediately after surgical implantation to improve host-graft integration. A class of mineralized collagen scaffolds has recently been identified to promote osteogenesis of bone marrow and adipose-derived mesenchymal stem cells both…
Researchers have developed a novel method to enhance the regenerative potential of adipose-derived stem cells (ASCs) within mineralized collagen scaffolds. This innovation could revolutionize the reconstruction of critical-sized craniomaxillofacial defects by accelerating proangiogenic processes after surgical implantation. The team achieved this by employing a glycoengineering approach to create engineered ASCs (eASCs) with an azido sugar tag on their cell membrane.
This tag enables selective targeting using DBCO-tagged biomolecules, allowing for the boosting of eASCs activity. The study explored the effects of azido sugar labeling conditions on labeling efficiency and found that proliferation and pro-regenerative gene expression activity of eASCs in mineralized collagen scaffolds were largely comparable to conventional hASCs.
Furthermore, the researchers demonstrated that molecular cargo functionalized with dibenzocyclooctyne (DBCO) could be conjugated to azido groups on eASCs via click chemistry. The findings suggest that metabolically labeled eASCs hold promise for selective delivery of activity-inducing bioactive cues, ultimately enhancing the regenerative potential in these scaffolds.
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