Click-Prep: An Interactive Data Preparation Tool for Click-qPCR
Click-qPCR is a browser-based application for relative qPCR analysis that requires a tidy-format CSV file containing four columns: sample, group, gene, and Cq. Preparing this input from qPCR instrument output typically requires manual reformatting and calculation of mean Cq values for technical replicates. To simplify this process, we developed Click-Prep…
The Click-qPCR application is a browser-based tool for relative quantitative PCR analysis. It necessitates a tidy-format CSV file with four columns: sample, group, gene, and Cq. Creating this input from qPCR instrument output usually involves manual reformatting and computation of mean Cq values for technical replicates. To streamline this procedure, researchers have created Click-Prep, an interactive web-based application tailored for generating Click-qPCR input files.
Click-Prep accepts CSV, TXT, TSV, and XLS/XLSX files, allowing users to bypass instrument-generated metadata rows, interactively map columns, and manually assign experimental groups. Users can examine technical-replicate measurements, exclude rows based on established quality-control standards, and compute mean Cq values for each sample-group-target combination.
The tool flags missing or nonnumeric Cq values for review and requires resolution before the mean is calculated. Additionally, Click-Prep can merge compatible formatted CSV files, such as datasets from separate qPCR plates. The final dataset is exported as a standardized CSV file containing the required four fields for Click-qPCR.
By consolidating these operations into a guided browser-based workflow, Click-Prep enables users to efficiently and uniformly prepare Click-qPCR input files without the need for programming skills.
Written by urgent.news from bioRxiv's reporting — not their text. Machine-written — may contain errors; check the original before relying on it.
