CD8+ T CELLS ASSOCIATE WITH FORMING GLANDULAR NODULES IN TOXOPLASMA GONDII-INDUCED PROSTATIC HYPERPLASIA AND HUMAN BPH
Background: Chronic inflammation is the most common histological feature in Benign Prostatic Hyperplasia (BPH), and T cells are a key component of immune infiltrate. Advanced BPH is commonly associated with the formation of nodules, but it remains unclear whether a link exists among T cell infiltration, nodular development, and BPH progression. Using a Toxoplasma gondii (T. gondii) model and…
Chronic inflammation is a prevalent histological trait in Benign Prostatic Hyperplasia (BPH), with T cells playing a crucial role in the immune response. As BPH advances, the formation of nodules is often observed, yet the connection between T cell infiltration, nodule development, and BPH progression remains unclear. Utilizing a Toxoplasma gondii (T. gondii) model and human samples, researchers examined the types of T cells present during prostatic hyperplasia and their relationship with nodule formation in the prostate.
Male CBA/j mice were intraperitoneally infected with T. gondii parasites, and flow cytometry was employed to assess the quantity of CD4+ and CD8+ T cells in the prostate. Histological analysis was conducted to evaluate microglandular hyperplasia (MGH), while immunofluorescence was utilized to quantify and examine the distribution of CD4+ and CD8+ T cells, comparing them to human BPH tissue samples.
The findings indicated that T. gondii infection in male mice led to an acute increase in both CD4+ and CD8+ T cells within the prostate, with CD8+ cells persisting chronically. Hematoxylin and eosin (H&E) staining confirmed the presence of glandular nodules at this stage. Immunofluorescence revealed that CD8+ cells were located near the formation of glandular nodules compared to non-nodular glands.
Additionally, a greater number of CD8+ cells were found in non-nodular glands within nodular BPH tissue as opposed to non-nodular BPH tissue.
Moreover, the study discovered a higher prevalence of CD8+ cells in patients with T. gondii IgG antibodies compared to those without the antibodies. All T. gondii IgG+ patients exhibited nodular BPH, while all but one IgG- patient had non-nodular BPH. This research, the first to investigate the presence and location of CD4+ and CD8+ T cells within nodular and non-nodular BPH glands, establishes an association between the presence of CD8+ T cells and nodular progression.
This connection was observed in both human prostate tissue and the T. gondii model. Consequently, CD8+ T cells may contribute to the advancement of nodular BPH, and T. gondii infection may facilitate this CD8+ T cell-mediated response.
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