Virus-like particle-delivered base editor collection to expand the genome engineering toolbox
Virus-like particles (VLPs) enable transient, non-integrating delivery of CRISPR-Cas9 ribonucleoprotein cargo. Although VLPs have been reported for efficient DNA editing via base editors RNP delivery, the diversity of base editors tested as VLPs remains limited. We generated and benchmarked a panel of 12 base editors on the v5 eVLP backbone, targeting three genomic loci (HEK3, B2M, PDCD1) across…
Virus-like particles (VLPs) are being utilized to deliver CRISPR-Cas9 ribonucleoprotein cargo for transient, non-integrating DNA editing. Despite VLPs showing promise in base editor delivery, the range of base editors tested in VLPs has been relatively narrow. To address this, researchers have created and assessed a collection of 12 base editors on the v5 eVLP scaffold, targeting three genomic sites (HEK3, B2M, PDCD1) in LentiX-293T cells using five different VLP concentrations.
The editing efficiency varied with the dosage and editor class, with PAM-flexible variants demonstrating lower efficiency compared to NGG-restricted editors. Additionally, dual-function SPACE base editors exhibited reduced performance. The study further delved into the position-specific editing efficiencies and outcomes of the base editor VLP collection, which revealed a broad spectrum of mutation types that can be achieved using these base editors.
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