Probing relaxed myosin states in hypertrophic cardiomyopathy by second harmonic-generation microscopy
This study explores the use of polarized second-harmonic generation (pSHG) to investigate myosin conformation in the relaxed state, differentiating between the actin-available, disordered (ON) state and the energy-conserving, ordered (OFF) state. By shifting the ON/OFF equilibrium using both physical and chemical manipulations, we demonstrate the sensitivity of pSHG in quantifying the ON/OFF…
We haven't written up this one. eLife has the full story — the link below goes straight to it.
