An anaerobic cell-free protein synthesis formulation for the in vitro study of oxygen sensitive proteins
Anaerobic microbes harbor unique enzymes and metabolic pathways with potential biotechnological applications, yet these microbes remain difficult to study and engineer. Research is limited by the labor-intensive workflows required to maintain anoxic environments and the lack of genetic tools available for non-model anaerobes. Here, we present an anaerobic cell-free protein synthesis (CFPS)…
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