Moderately acid-tolerant Nitrospira strains obtained by single-colony isolation on plates
Nitrospira are difficult to cultivate, and pure cultures are mainly obtained by dilution-to-extinction combined with other methods, a process that usually takes years to over a decade. Here, we isolated three Nitrospira strains from activated sludge and sediment within one year using conventional single-colony isolation. Pure cultures of two strains could grow and reform single colonies within…
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