Residual barcode-like sequences and barcode-associated artifacts after Dorado and Barbell preprocessing of metagenomic reads
Background Residual barcode-like sequences after preprocessing are a quality-control concern in multiplexed Oxford Nanopore Technologies (ONT) sequencing data, particularly in low-biomass, host-dominated samples such as blood. Using blood datasets spiked with a microbial mock community and prepared with the Native Barcoding Kit 96 V14 (SQK-NBD114.96), we compared Dorado 1.1.1, Dorado 2.0.0, and…
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