Unilateral naris occlusion with custom-made plugs for reversible olfactory deprivation in juvenile and adult mice
Deprivation paradigms that reduce environmental input have been successfully applied across multiple species and sensory modalities, including vision, audition, somatosensation, and olfaction in order to understand how neurons and circuits respond to loss of sensory input. One notable advantage of studying sensory deprivation in the rodent olfactory system is the ease and control with which smell…
Deprivation techniques that diminish environmental stimuli have been effectively utilized across various species and sensory systems, such as vision, hearing, touch, and smell, to study how neurons and neural networks react to the absence of sensory input. One significant benefit of investigating sensory deprivation in rodent olfactory systems is the simplicity and precision with which smell loss can be induced.
Numerous methods for inducing olfactory deprivation are available, which either permanently block a nostril via chemical removal or surgical procedures, or temporarily block it using reversible techniques like unilateral naris occlusion with custom-made nose plugs. This article presents a comprehensive overview of this well-established procedure, encompassing its assembly from readily available materials, execution, validation, and consistency in post-weaning mice, thereby offering a comprehensive and repeatable framework for regulated, reversible olfactory deprivation.
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