Long read sequencing reveals changes in measured isoform structure and abundance due to RNA processing
Oxford Nanopore Technology enables direct RNA sequencing without reverse transcription (RT) and cDNA sequencing without PCR amplification, allowing investigation of how RNA preparation protocols bias sequencing data. PCR amplification is known to introduce biases related to expression levels, transcript lengths, and GC content. However, biases introduced by RT have been described more recently…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
