NPlasmid-seq: Cas9-encoded terminal signatures enable molecule-level nanopore quality control of vector and IVT-template plasmids
Consensus-based plasmid quality control can overlook structural heterogeneity within a preparation. We developed NPlasmid-seq, a Cas9-guided nanopore workflow that encodes sample identity through paired terminal signatures generated at predefined backbone positions, without sample-specific oligonucleotide barcodes. We cleavage-validated 24 sgRNAs. In five representative assigned read bins, each…
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