Marked Mutants Allow for Rapid Non-Invasive Genotyping of Zebrafish by Visual Inspection Alone
Genetic analysis in zebrafish relies heavily on invasive, labour-intensive molecular genotyping. This limits throughput and phenotypic analysis, particularly for homozygous mutants that lack readily detectable phenotypes at early developmental stages. To overcome these limitations, we adapted CRISPR-mediated knock-in strategies to insert complete secondary marker cassettes (including promoter,…
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