Light-sheet fluorescence microscopy of optically cleared human head-and-neck organs: three-dimensional tissue assessment and histological correlation
Conventional histology provides detailed cellular information but only a partial view of the three-dimensional (3D) organisation of head-and-neck tissues. We investigated whether optical tissue clearing combined with light-sheet fluorescence microscopy (LSFM) could visualise the microanatomy of large head-and-neck specimens and relate volumetric features to conventional histology. Non-neoplastic…
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