Single-molecule nascent RNA profiling reveals multiple layers of co-transcriptional splicing and their reprogramming by cold in Arabidopsis
In eukaryotes, RNA polymerase II (Pol II) transcription is closely coupled to RNA processing, yet how polymerase progression coordinates multiple processing events within individual nascent transcripts, particularly in response to environmental cues, remains poorly understood. Here, we developed plant polymerase intact nascent transcript sequencing (pPOINT-seq), a long-read sequencing approach…
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