Genomic and environmental determinants of circular single-stranded DNA production in engineered E. coli
Single-stranded DNA with hundreds to thousands of bases is a critical reagent in biotechnology. While traditional chemical synthesis is not suitable for efficient production of such long oligonucleotides, M13 bacteriophage offers a scalable pathway towards custom, long, single-stranded DNA production. However, conventional helper plasmid-phagemid systems suffer from batch-to-batch variability and…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
