A quantitative three-dimensional bone mineralisation assay using human dental pulp stem cell spheroids
Matrix mineralisation in three-dimensional (3D) human cell culture is still assessed predominantly by terminal histochemical staining. Alizarin Red S and Von Kossa are destructive, semi-quantitative, and require fixation and dye extraction before a number can be obtained, which precludes longitudinal measurement and makes miniaturised multiwell formats impractical. We describe a scaffold-free…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
