Scalable variant-to-function screens using prime editing perturb-seq
Linking genetic variants to their functional consequences at scale remains a major challenge in human genetics. Perturb-seq is limited in the variants it can install, while prime editing has largely been restricted to simpler mechanistic readouts. Here, we introduce prime editing perturb-seq (PEP-seq), which links programmed mutations to transcriptomic states. We designed 2,164 prime editing…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
