Compact CRISPR-based genetic circuitry using a miniature genome-integrated Cas12m protein
CRISPR-Cas systems can be repurposed as programmable transcription factors, offering a virtually limitless supply of orthogonal regulators for building genetic circuits. However, the commonly used dCas9-based systems place a heavy burden on the host, are slow to release their target, and suffer interference between guide RNAs as they compete for a shared pool of Cas protein. Here we show that…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
