Application of the ORBIT Protocol for Targeted Gene Deletion to Mycobacterium abscessus
Mycobacterium abscessus (Mab) is an emerging opportunistic pathogen characterized by extensive intrinsic drug resistance, underscoring the need for precise genetic tools to define mechanisms of pathogenicity and persistence. The ORBIT (oligonucleotide-mediated recombineering followed by Bxb1 integrase targeting) system developed by the Sassetti group enables precise gene deletions but has…
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