Development of a high throughput continuous fluorescence anisotropy-based assay to analyse peptidoglycan-digesting enzymes
Bacterial cell wall processing enzymes have a central role in the control of the construction, remodelling and breakdown of the peptidoglycan wall that protects bacteria from osmotic rupture. Many of these enzymes have biologically essential functions and exert mechanistically diverse activities on a complex polymer substrate. Despite this biological importance they are relatively understudied…
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