Force-induced unfolding versus unbinding of a cellulosomal carbohydrate-binding module from cellulose interface
Carbohydrate-binding modules (CBMs) anchor microbial cellulosomes to cellulose surfaces, aiding biomass degradation under mechanical stress. To understand how mechanical pulling affects this process, we combine steered molecular dynamics (SMD) simulations with acoustic force spectroscopy (AFS) to investigate Clostridium thermocellum CBM3a unbinding from cellulose under different pulling…
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