P2K1 P2K2 Coordinate Early, Structure-Specific Mechanical Adaptations to Osmotic and Cell Wall Stress in Arabidopsis Roots
The cell wall and plasma membrane (PM) form the interface between a plant cell and its environment yet how their mechanical properties change in response to stress is poorly understood. Here we investigate how the mechanical properties of the cell wall, plasma membrane, and vacuole change in vivo in Arabidopsis thaliana roots in response to hyperosmotic stress and cell wall damage (CWD) using a…
Arabidopsis thaliana roots demonstrate structural adaptations to osmotic and cell wall stress, focusing on the mechanical properties of the cell wall, plasma membrane (PM), and vacuole. Using a solvatochromic dye, molecular rotors, and Brillouin microscopy, researchers observe dynamic modifications in the PM's mechanical properties upon hyperosmotic stress and cell wall damage (CWD). The cell wall experiences limited changes, while the vacuole adjusts after several hours.
The p2k1 p2k2 double mutant, which lacks receptors for extracellular ATP perception, exhibits impairment in sorbitol-induced effects on PM mechanostasis and early isoxaben-induced enhancements in PM order. These findings suggest that P2K1 and P2K2 are crucial in regulating adaptive changes in the PM's mechanical properties due to hyperosmotic stress and CWD.
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