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Evaluation and primer for using DeepLabCut to track small animals in large spaces

DeepLabCut (DLC), a machine learning-based pose estimation tool, has dramatically increased the throughput of behavioral tracking. Yet its implication remains limited by lack of validation data from diverse scenarios and users' unfamiliarity with the machine learning workflow. Here, we provide a practical primer on DLC terminology and workflow and performance evaluation of DLC in a novel scenario…

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