In silico subtractive genomics and reverse vaccinology approach reveal outer membrane proteins OMP47 and LptD as dual-purpose drug and vaccine targets in Pasteurella multocida Pm70
Drug targets and vaccines for bacterial diseases are challenging to develop. Computational subtractive genomics and reverse vaccinology offer a viable substitute for conventional drug and vaccine development approaches. In recent years, Pasteurella multocida, a Gram-negative pathogen responsible for severe infections in humans along with major diseases in livestock and poultry, has developed…
In the battle against Pasteurella multocida infections, researchers have turned to computational methods to identify potential drug and vaccine targets. The study focused on the complete proteome of Pasteurella multocida Pm70, uncovering 446 proteins that have no human protein counterparts. From this pool, 129 proteins were deemed essential for the pathogen's survival, making them prime candidates for drug development.
Further examination of these proteins revealed two outer membrane proteins, OMP47 and LptD, as promising targets for both therapeutic intervention and vaccine creation. Following antigenicity analysis, both proteins emerged as highly suitable vaccine candidates, exhibiting excellent antigenicity scores. Researchers then mapped high-confidence B- and T-cell epitopes from these targets, assembling them into a comprehensive multi-epitope vaccine (MEV) that is both non-toxic and non-allergenic to humans.
Although further experimental validation is needed, this study lays the groundwork for developing novel therapeutics and vaccines to combat the increasing cases of Pasteurella multocida infections.
Written by urgent.news from bioRxiv's reporting — not their text. Machine-written — may contain errors; check the original before relying on it.
