Benchmarking interface confidence as a selection criterion for computational nanobody design against survivin's BIR-domain groove
Designed binders are routinely filtered on predicted interface confidence, which reports how self-consistently a model places an interface, not where it lies. We designed 200 nanobody loops against the survivin BIR-domain groove, co-folded 196, and checked epitope contact in every predicted complex. Confidence showed no detectable association with epitope engagement ({rho} = +0.100, 95% CI -0.04…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
