3D-printed inserts bring single-objective light sheet microscopy to commercial sample chambers
Light sheet microscopy allows biologists to selectively light up a thin slice of a sample. This method can improve image contrast by reducing background light while remaining gentle on the sample, reducing photodamage and photobleaching. Rice University's Anna-Karin Gustavsson has published a method in Nano Letters that expands the use of light sheet microscopy to work with a single objective in…
Light sheet microscopy is a method that selectively lights up a thin slice of a sample, improving image contrast while being gentle on the sample and reducing photodamage. Rice University's Anna-Karin Gustavsson has developed a method in Nano Letters that allows light sheet microscopy to be used with a single objective in commercially available sample chambers, eliminating the need for two objectives or specialized chambers.
The team adapted light sheet microscopy to work in microfluidic chips, creating a single-objective approach that involves reflecting the light sheet into the sample inside the chip. Gustavsson and her team then 3D nanoprinted noncytotoxic inserts to generate a mirror for light sheet reflection, allowing the method to be used in standard sample chambers without disrupting existing sample preparation workflows.
This technique reduces background fluorescence, photobleaching, and photodamage, enabling clearer imaging of individual molecules with less sample damage.
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