CRISPR RNA-Guided Integrase Enables Targeted Genome Engineering and Prophage Functional Genomics in Plant-Pathogenic Pseudomonas

Pseudomonas species include important plant pathogens responsible for substantial agricultural losses. Although CRISPR-INTEGRATE has been applied for genome engineering in medically relevant Pseudomonas, its utility in plant-pathogenic Pseudomonas has not been established. Here, we demonstrate CRISPR-INTEGRATE, an RNA-guided Tn7-like transposition system that enables targeted chromosomal…

biorxiv.org ·1d ago

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