RNA self-association limits the removal of double-stranded RNA by affinity chromatography
Double-stranded RNAs are inflammatory byproducts of in vitro transcription of single-stranded RNA. Here we investigate removal of dsRNA byproducts using dsRNA affinity chromatography (dsRNA-AC) and two mRNA molecules with contrasting biophysical properties: 1) GFP, and 2) high dsRNA wild-type firefly luciferase (fLucWT). For GFP mRNA, dsRNA reduction was strong, and mRNA recovery was high. In…
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