Processing and Analyzing High-Throughput Microfluidic Enzymology Data: A Practical Guide To Rate Fitting and Quality Control
High-throughput enzymology enables quantitative characterization of enzyme function across hundreds to thousands of sequence variants and experimental conditions. Nevertheless, the scale and complexity of these datasets create substantial challenges for analysis and quality control. High-Throughput Microfluidic Enzyme Kinetics (HT-MEK), for example, generates large microscopy datasets that must…
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