Impact of noise on optical redox ratio estimation
Optical redox ratio (ORR) is a commonly used metric to quantify the metabolic state of biological samples based on autofluorescence of NAD(P)H and FAD. Despite its relevance and popularity, little work has been done to quantify the estimation error of ORR, especially in low-photon count data. Here, we provide an analytical solution to determine ORR variability in the presence of shot noise and…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
