Updated Transposable Element Libraries for Drosophila melanogaster in Dfam 4.0
Drosophila melanogaster repeatome, comprising roughly 20% of the genome, is characterized by a large fraction of active TE families counterbalanced by efficient purifying selection. Consequently, many TE families persist at low copy numbers and are frequently population specific. Hybridization and horizontal transfer provide new families, which can spread through natural populations within…
Glycans play a crucial role in various physiological processes. Despite their growing importance and potential for translation, there is still a lack of tools to study glycosylation in living organisms. In this study, researchers introduce FlyMOE, a novel chemical-genetic platform designed for targeted glycoprotein profiling in Drosophila melanogaster. The platform involves transgenic flies with the ability to biosynthetically install bioorthogonally-tagged monosaccharides into the cellular glycoproteome.
The genetic flexibility of Drosophila allows for the application of FlyMOE across different developmental stages, genetic drivers, and glycosyltransferase enzymes. Utilizing FlyMOE, the researchers generated a comprehensive overview of the glycoproteome in various Drosophila tissues and embryos through mass spectrometry glycoproteomics. Additionally, they investigated the substrates of individual members of the PGANT glycosyltransferase family in vivo.
By employing FlyMOE, researchers can now conduct detailed investigations into the glycoproteome of Drosophila and other genetically modifiable in vivo model systems. This chemical-genetic approach offers a promising tool for further understanding the role of glycans in physiology and their potential translational applications.
Written by urgent.news from bioRxiv's reporting — not their text. Machine-written — may contain errors; check the original before relying on it.
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