Shaping cryogenic 3D volume imaging by pFIB ion species and milling voltage
Cryogenic plasma focused ion beam-scanning electron microscopy (cryo-pFIB-SEM) enables nanoscale volume imaging of vitrified cells and tissues, but the fidelity of each newly exposed block-face depends on how ions interact with compositionally heterogeneous biological material. Using xenon, argon, oxygen, and nitrogen plasma beams across a 30-2 kV voltage range, we examined how ion species and…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
