CRISPR/Cas9 Optimization Using Electronic Genome Mapping: Potential Role for Studying Human Genetic Disease
CRISPR Cas9's ability to bind targeted and non-targeted sites and its functional interactions with other proteins and domains have been studied using many technologies. These studies are often limited by using oligos as substrates or by a reliance on predicted binding sites. We show how electronic genome mapping (EGM) with its ability to detect single-molecule DNA nicks can determine how gRNA…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
