An Ultrasensitive Luciferase Reporter Reveals Low-Level Extrahepatic mRNA Expression for Enhanced Apparent Biodistribution of Lipid Nanoparticles
Reporter mRNAs are widely used to screen lipid nanoparticle (LNP) formulations and characterize functional mRNA expression in vivo, yet conventional firefly luciferase (FLuc) imaging fails to detect low-level expression. Consequently, weak or absent FLuc signal can be misinterpreted as failed LNP delivery or translation, potentially excluding formulations and overlooking extrahepatic or…
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