First evidence for TIGR-Tas activity in a photosynthetic eukaryote
TIGR--Tas systems are a recently described class of RNA-guided DNA-targeting systems whose activity has been demonstrated in bacterial and human cells, but not in photosynthetic eukaryotes. Here we tested TIGR--Tas in the green alga Chlamydomonas reinhardtii. Recombinant TaTas and ParTas assembled with in vitro-transcribed tigRNAs cleaved target DNA in a concentration-dependent manner, and…
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